供应美国NIST临床检验材料牛血清白蛋白标准物质SRM 927f

供应美国NIST临床检验材料牛血清白蛋白标准物质SRM 927f

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This Standard Reference Material (SRM) is intended primarily for use in the standardization of procedures employed in clinical analyses for total serum protein, for critical evaluation of daily working standards used in these procedures,and as a reference standard for assays of total protein by colorimetric methods. This SRM is a solution (mass fraction approximately 7 %) of known protein concentration and purity. The total protein content of this SRM was determined using the biuret reference method [1] that is recommended for use in standardizing laboratory-prepared protein solutions and “normal” serum pools. Such standardized “normal” sera could then be used to calibrate refractometers or other instruments for serum protein estimations. SRM 927f may also be used for other procedures, such as gel diffusion, amino acid analysis, electrophoresis, nitrogen assays, or other tests that require well-characterized protein for calibration or evaluation. A unit of SRM 927f consists of 10 ampoules, each containing approximately 2.2 mL of solution. Measurements of the bovine serum albumin (BSA) concentration were made using two independent methods including amino acid analysis and the biuret method. The results from the two approaches are reported as follows: 1) certified BSA concentration by amino acid analysis and 2) reference BSA concentration by the biuret method. 美国NIST临床检验材料

本标准参考物质（SRM）主要用于血清总蛋白临床分析程序的标准化，用于这些程序中使用的日常工作标准的关键评估，以及作为比色法测定总蛋白的参考标准。该SRM是已知蛋白质浓度和纯度的溶液（质量分数约为7%）。该SRM的总蛋白质含量是使用缩二脲参考方法[1]测定的，该方法建议用于标准化实验室制备的蛋白质溶液和“正常”血清库。这种标准化的“正常”血清可以用于校准折射计或其他用于血清蛋白估计的仪器。SRM 927f也可用于其他程序，如凝胶扩散、氨基酸分析、电泳、氮测定或其他需要良好表征的蛋白质进行校准或评估的测试。SRM 927f的一个单元由10个安瓿组成，每个安瓿含有约2.2毫升溶液。牛血清白蛋白（BSA）浓度的测量使用两种独立的方法进行，包括氨基酸分析和缩二脲法。两种方法的结果报告如下：1）通过氨基酸分析鉴定的BSA浓度和2）通过缩二脲法测定的参考BSA浓度。 临床检验材料牛血清白蛋白标准物质

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